Journal: bioRxiv
Article Title: Regulation of input excitability in human and mouse parvalbumin interneurons by Kir potassium channels
doi: 10.1101/2025.04.11.648314
Figure Lengend Snippet: a) Analysis of mRNA in patch-sequenced human and mouse Pvalb neurons. The heatmap presents the mRNA levels (transcripts per million [TPM]) in individual human (n = 21) and mouse (n = 10) neurons for the genes KCNJ1–16 encoding Kir family channels, as well as the housekeeping genes GAPDH and ACTB . The abscissa presents Pvalb neurons with their subtype identity defined by their gene expression pattern in the Allen Institute Neocortical Neuron Type Identification System (Pvalb neuron type identity codes shown in the abscissa). Human and mouse cells exhibit mRNA of KCNJ3 (Kir3.1) and KCNJ6 (Kir3.2), and also KCNJ9 (Kir3.3) which is higher in mouse. In addition, the human cells express KCNJ4 (Kir.2.3). p-values for the difference between human and mouse are presented on the left (MANOVA with Bonferroni’s post hoc test) with significance difference denoted by red text. b) The heatmap presents the Allen Institute data for the mean KCNJ gene mRNA level (TPM) in the different Pvalb neuron subtypes in humans and mice. The mRNA levels are high in human and mouse cell types for KCNJ3 and KCNJ6 . Mouse cells have higher expression of KCNJ4 , KCNJ9, KCNJ11 , and KCNJ12 (MANOVA with Bonferroni’s post hoc test). c) The plot presents the lack of an association between the Kir channel gene mRNA level (TPM, summed for all KNCJ types) and input rectification ratio (r Rin ). r Rin = 1 indicates the lack of a Kir channel effect (Spearman’s correlation test).
Article Snippet: For dSTORM microscopy rabbit Anti-GIRK1 polyclonal was used (1:200, #APC-005, https://www.alomone.com/p/anti-kir3-2-girk2/APC-006 ).
Techniques: Gene Expression, Expressing